Jason Euell's U23s host Bristol City at Dartford's Princes Park on Monday afternoon (2pm).
The young Addicks will be hoping to build on their 2-1 victory at Crystal Palace last week and stretch their unbeaten league run to four games.
Charlton currently sit in fourth position in Professional Development League 2 - South, two points above Monday's opponents who sit in seventh.
Supporters are welcome to attend all of the Addicks' development squad matches at Princes Park. Entrance is free for Valley Gold members and season-tickets holders, or £3 adults and £1 juniors on the door.
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