Ashley Maynard-Brewer and Nathan Harness have been recalled from their loan spells with Gillingham and Bromley.
Maynard-Brewer joined the League Two side in June but suffered a dislocated shoulder during their pre-season friendly against Dover Athletic just weeks later.
The academy graduate, who returned to Charlton for his rehabilitation period and recently featured for Hamza Serrar's U21s against Bristol City, will wear the number 31 shirt this season.
Harness, meanwhile, will wear number 30 following a short spell with National League side Bromley.
Charlton fans can support the academy by signing up to the Valley Gold scheme. Valley Gold enables fans to support the club's academy in unearthing the talents of tomorrow, as well as giving them the chance to win cash prizes.